I-Reticulocyte Daye Ye-Sysmex Hematology Analyzers

I-Reticulocyte Dye ye-Sysmex Hematology Analyzers ihlinzeka nge-fluorescent nucleic acid staining yamapulatifomu e-semiconductor laser flow cytometry. I-reagent ewuketshezi ingena kumaseli egazi ane-nucleated, ama-reticulocyte, nama-platelet ukuze abophe ngokukhethayo nge-RNA ne-DNA ye-intracellular, akhiqize i-fluorescence ethile eseceleni kanye namasiginali wokuhlakaza ukukhanya kuwo wonke amashaneli okuthola ulwazi. Yakhelwe ukuhlanganiswa okuqondile namasistimu e-fluorescence azinzile, umsindo ongemuva ophansi, kanye nenqwaba ekwazi ukukhiqiza kabusha-ukuze{3}}kusebenze okuningi kuzilungiselelo zaselabhorethri yomtholampilo.

Ama-automated hematology dye reagents amamaki e-fluorescent awuketshezi aklanyelwe ukugeleza-i-cytometry-asekelwe ezinkundleni zokuhlaziya amaseli. Ngokungafani namabala e-blood smear endabuko asetshenziswa ku-microscopy ekhanyayo, lab' odayi abawuketshezi bangena kumaseli okuqondiwe kuwo ngokusebenzisa i-membrane permeabilization ukuze ilebula ama-nucleic acid e-intracellular. Ngaphansi kwe-semiconductor laser excitation ngaphakathi kweseli lokugeleza kokubona, izakhiwo zeselula ezifakwe ilebuli zikhipha amasignali aseceleni e-fluorescence namasignali e-scatter yokukhanya, okuvumela okokubona kwesistimu ukuthi kukhiqize ama-scattergram amabili anembile kanye nokusabalalisa kweqoqo lamaseli.

Ekuhlaziyweni kwe-hematology ye-optical, odayi be-fluorescent bavumela ukubandlululwa kwamaseli okuhlukile ngokukala ukuminyana kwe-nucleic acid ye-intracellular:

Umehluko Weseli Elimhlophe Legazi:Ihlukanisa ama-lymphocyte, ama-monocyte, ama-neutrophils, ama-eosinophil, nama-basophil ngokusekelwe kuzilinganiso ze-RNA/DNA kanye nesakhiwo se-cytoplasmic.

Ukuhlonza Iseli Okungajwayelekile Nengavuthiwe:Ihlonza amangqamuzana okuqhuma kanye nama-granulocyte angavuthiwe ngokusebenzisa amandla okukhishwa kwe-fluorescence aphakeme.

Ukubalwa kwe-Reticulocyte:Ikala i-ribosomal RNA eyinsalela kumaseli abomvu egazi amancane ukuze ihlole umsebenzi we-erythropoietic womnkantsha.

Ukubala kwePlatelet yokubona:Ixazulula ama-platelet amakhulu futhi ihlukanise izingcezu zamangqamuzana egazi abomvu kuma-platelet kusetshenziswa i-nucleic acid fluorescence gating eqondiwe.

Udayi we-fluorescent usebenza eziteshini ezithile ezizenzakalelayo zokubona:

Isiteshi se-WNR:Ibala amangqamuzana egazi amhlophe futhi ihlukanise amaseli abomvu egazi nge-nucleic acid staining.

Isiteshi se-WDF:Ihlukanisa i-leukocyte subpopulations evuthiwe futhi ithola ama-granulocyte angavuthiwe isebenzisa i-fluorescence eseceleni kanye nemingcele yokuhlakaza emaceleni.

Isiteshi se-WPC:Izikrini zamangqamuzana okhokho kanye nama-lymphocyte angajwayelekile asekelwe kubuningi benuzi kanye nomehluko wokungena kwe-membrane.

Isiteshi se-RET:Ikala izingxenyana ze-reticulocyte namapharamitha amaseli abomvu egazi ngokusebenzisa amandla e-RNA fluorescence.

Isiteshi se-PLT:Ilebula ama-nucleic acid e-platelet ukuze asuse ukuphazamiseka kwamangqamuzana egazi abomvu e-microcytic ngesikhathi sokubala ngokubona.

Yakhelwe ukuqondanisa okuqondile kokusebenza nezinkundla ze-hematology ezizenzakalelayo zisebenzisa i-semiconductor laser flow cytometry:

Uchungechunge lwe-XN:I-XN-1000, XN-2000, XN-3000, XN-9000, XN-L Series (XN-350, XN-450, XN-550)

Uchungechunge lwe-XS:XS-500i, XS-800i, XS-1000i

Uchungechunge lwe-XT:XT-1800i, XT-2000i, XT-4000i

XE Series:XE-2100, XE-5000

I-WNR Channel Staining (STAC-DYE-SYSWNR):Ivumela ulwelwesi lwamaseli ukuthi luvumele ukungena ngokushesha kukadayi we-nuclear, luhlukanise i-NRBC nuclei engaguquki kumaseli egazi amhlophe ukuze uthole i-NRBC % enembile kanye nokukhiqizwa kwesibalo se-WBC.

I-WDF Channel Staining (STAC-DYE-SYSWDF):Ama-intracellular nucleic acids we-leukocyte ukukhiqiza amaqoqo e-scattergram ahlukene ama-neutrophils, ama-lymphocyte, ama-monocytes, nama-eosinophils.

I-WPC Channel Staining (STAC-I-DYE-SYSWPC):Ikhetha amabala okuqhuma amaseli nama-lymphocyte angajwayelekile asuselwe ekwakhekeni kwe-lipid nokuminyana kwe-nuclear ekuhlabeni umkhosi kweseli kwesibili.

I-RET Channel Staining (STAC-DYE-SYSRET):Ibophezela ku-RNA eyinsalela kumaseli egazi abomvu angavuthiwe, okuvumela ukubalwa kwesamba sephesenti le-reticulocyte, ukubala okuphelele, nengxenye ye-reticulocyte engavuthiwe.

I-PLT Channel Staining (STAC-DYE-SYSPLT):Ilebula ama-platelet nucleic acids ukuze kuvinjelwe ukuphazamiseka kweseli elibomvu legazi le-microcytic phakathi{0}}nokulinganisa okuphansi kwe-optical platelet.

Ukucushwa Kwephakheji:

• Amakhasethi e-Cartridge: 2 × 12 mL
• Isixazululo sebhodlela: 1 × 50 mL, 1 × 100 mL, 1 × 500 mL

Izinga Lokushisa Lesitoreji:2℃kuya ku-30 ​​degree (Vikela ekukhanyeni okuqondile kanye nokubanda)
Impilo Yeshelufu Engavuliwe:Izinyanga ezingu-18
Ebhodini-Ukuzinza:Izinsuku ezingama-60 ezimeni zaselabhorethri ezizungezile

Izixazululo ze-Hematology Diluent:Izixazululo ze-isotonic buffer ezigcina ubuqotho bevolumu yeseli kanye nokulawula ukuqhutshwa koketshezi ngesikhathi sokulinganisa ngokomzimba.
Ama-reagents e-Lyse we-Leukocyte Differential:Izixazululo ezisebenzayo ezikhetha ngokukhetha amaseli abomvu egazi ukuze kulungiswe amaseli amhlophe egazi ukuze ahlaziywe.
Izixazululo zokuhlanza i-Analyzer:Uketshezi oluwashwayo lwe-Enzymatic oludizayinelwe ukusula ukunqwabelana kwamaprotheni nezinsalela zikadayi emigqeni yangaphakathi ye-fluidic namaseli agelezayo abonakalayo.
I-Concentrated Sheath Fluids:Uketshezi lwe-Hydrodynamic olwakhelwe ukubeka isampula phakathi kwemifudlana ngaphakathi kwamagumbi okubona amehlo.

Amashadi ashisayo: Udayi we-reticulocyte wabahlaziyi be-hematology be-sysmex, idayi yaseChina ye-reticulocyte yabakhiqizi be-sysmex hematology analyzers, abahlinzeki